Mycobacterium Tuberculosis INH Mutation
ingoa hua
HWTS-RT137 Mycobacterium Tuberculosis INH Kete Tirohanga Whakarereketanga (Ana Rewa)
Epidemiology
Ko te Mycobacterium tuberculosis, ko te Tubercle bacillus (TB), ko te huakita pathogenic e puta ai te mate kohi. I tenei wa, ko nga raau taero anti-tuberculosis e whakamahia nuitia ana ko te INH, rifampicin me te hexambutol, me etahi atu. Ko te mycobacterium tuberculosis e whakawhanake ana i te aukati i nga raau taero ki nga raau taero anti-tuberculosis, e kawe mai ana i nga wero nui ki te aukati me te maimoatanga o te mate kohi.
Hongere
| FAM | MP waikawa karihi |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | te huhu |
| CV | ≤5% |
| LoD | He 2x103 huakita/mL te tepe rapunga mo nga huakita INH momo mohoao, a ko te tepe kitenga mo nga huakita mutant he 2x103 huakita/mL. |
| Tauwhāiti | a. Karekau he tauhohenga whiti i waenga i te ira tangata, etahi atu mate pukupuku nontuberculous me te pneumonia i kitea e tenei kete.b. Ko nga waahi rereke o etahi atu ira aukati tarukino i roto i te momo mohoao Mycobacterium tuberculosis, penei i te rohe e whakatau ana i te rohe o te ira rifampicin rpoB, i kitea, a ko nga hua whakamatautau karekau he parenga ki te INH, e tohu ana kaore he tauhohenga ripeka. |
| Nga taputapu e tika ana | SLAN-96P Pūnaha PCR Wā-tūturuBioRad CFX96 Pūnaha PCR Wā-tūturuLightCycler480®Pūnaha PCR Wā-tūturu |
Rere Mahi
Mena ka whakamahia te Tohutono & Micro-Test General DNA/RNA Kit (HWTS-3019) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. raupapa, me te taapiri i te 10μL o te mana o roto ki roto i te mana kino, te tauira sputum tukatuka kia whakamatauria, me nga mahi o muri mai kia tino mahia kia rite ki nga tohutohu tangohanga. Ko te rōrahi tauira tangohia ko 200μL, me te rōrahi elution tūtohu ko 100μL.
