Te Huringa INH o te Mycobacterium Tuberculosis
Ingoa hua
Kete Kimi Huringa INH Mycobacterium Tuberculosis HWTS-RT137 (Pīkoi Rewa)
Te mate urutā
Ko te Mycobacterium tuberculosis, arā, ko te Tubercle bacillus (TB), te huakita mate e hua ai te mate kohi. I tēnei wā, ko ngā rongoā tuatahi e whakamahia whānuitia ana ko te INH, te rifampicin me te hexambutol. Ko ngā rongoā tuarua e whakamahia ana ko te fluoroquinolones, te amikacin me te kanamycin. Ko ngā rongoā hou kua whakawhanakehia ko te linezolid, te bedaquiline me te delamani. Heoi, nā te whakamahinga hē o ngā rongoā anti-tuberculosis me ngā āhuatanga o te hanganga pūtau o te mycobacterium tuberculosis, ka whakawhanakehia e te mycobacterium tuberculosis te ātete rongoā ki ngā rongoā anti-tuberculosis, ka puta he wero nui ki te aukati me te maimoatanga o te mate kohi.
Hongere
| FAM | Waikawa karihi MP |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | huware |
| CV | ≤5% |
| LoD | Ko te rohenga kitenga mō ngā huakita INH momo-mohoao ko 2x103 huakita/mL, ā, ko te rohenga kitenga mō ngā huakita whakarerekē ko 2x103 huakita/mL. |
| Te motuhaketanga | a. Kāore he tauhohenga whakawhiti i waenga i te ira tangata, ētahi atu huakita mycobacteria kore-koiora me ngā mate pukupuku niumōnia i kitea e tēnei kete.b. I kitea ngā wāhi whakarerekētanga o ētahi atu ira ātete rongoā i roto i te Mycobacterium tuberculosis momo-mohoao, pērā i te rohe whakatau ātete o te ira rifampicin rpoB, ā, kāore i whakaatuhia e ngā hua whakamātautau he ātete ki te INH, e tohu ana kāore he tauhohenga whakawhiti. |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha PCR Wā-Tūturu SLAN-96PNgā Pūnaha PCR Wā-Tūturu BioRad CFX96LightCycler480®Pūnaha PCR Wā-Tūturu |
Rere Mahi
Ki te whakamahia te Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. mō te tango, tāpirihia te 200μL o te tauira mana kino me te tauira huware tukatuka hei whakamātautau i roto i te raupapa, ka tāpirihia te 10μL o te mana ā-roto motuhake ki roto i te tauira mana kino, te tauira huware tukatuka hei whakamātautau, ā, me mahi tika ngā mahi e whai ake nei kia rite ki ngā tohutohu tango. Ko te rōrahi tauira i tangohia he 200μL, ā, ko te rōrahi wairewa kua tūtohutia he 100μL.
