Te Enterococcus ātete-Vancomycin me te Ira ātete-Rongoā

Whakaahuatanga Poto:

E whakamahia ana tēnei kete mō te kimi kounga o te enterococcus ātete-vancomycin (VRE) me ōna ira ātete-rongoa VanA me VanB i roto i te huware tangata, te toto, te mimi, ngā koroni parakore rānei.


Taipitopito Hua

Ngā Tohu Hua

Ingoa hua

HWTS-OT090-Kete Kimi Ira Ātete-Vancomycin me te Ātete-Rongoā (PCR Fluorescence)

Te mate urutā

Ko te Ātete ki te Rongoā e mōhiotia ana ko te ātete rongoā, e pā ana ki te ātete o ngā huakita ki te mahi a ngā rongoā patu huakita. Kia puta te ātete rongoā, ka tino heke te pānga chemotherapy o ngā rongoā. Ka wehea te Ātete ki te Rongoā ki te ātete ā-roto me te ātete whiwhi. Ka whakatauhia te ātete ā-roto e ngā ira ira huakita, ka tukuna iho i ia whakatipuranga ki ia whakatipuranga, ā, e kore e rerekē. Ko te ātete whiwhi nā te mea i muri i te pā atu ki ngā paturopi, ka whakarerekē ngā huakita i ō rātou ake ara pākia kia kore ai rātou e mate i ngā paturopi.

Ko ngā ira ātete vancomycin, arā, ko VanA me VanB, he ātete rongoā kua riro mai, ā, i roto i ēnei ko te VanA e whakaatu ana i ngā taumata teitei o te ātete ki te vancomycin me te teicoplanin, ā, he rerekē ngā taumata ātete a VanB ki te vancomycin, ā, he tairongo ki te teicoplanin. He maha ngā wā ka whakamahia te Vancomycin ki te rongoā i ngā mate huakita Gram-pai, engari nā te putanga mai o ngā enterococci ātete-vancomycin (VRE), inā koa ko te enterococcus faecalis me te enterococcus faecium, neke atu i te 90%, kua kawea mai he wero nui hou ki te rongoā haumanu. I tēnei wā, kāore he rongoā paturopi motuhake mō te rongoā i te VRE. Heoi anō, ka taea hoki e te VRE te tuku i ngā ira ātete rongoā ki ētahi atu enterococci, ki ētahi atu huakita Gram-pai rānei.

Hongere

FAM Enterococci ātete-vancomycin (VRE): Enterococcus faecalis me Enterococcus faecium
VIC/HEX Mana Whakahaere ā-roto
CY5 ira ātete vancomycin VanB
ROX ira ātete vancomycin VanA

Ngā Tawhā Hangarau

Rokiroki Wai: ≤-18℃
Te roa o te oranga 12 marama
Momo Tauira huware, toto, mimi, koroni parakore rānei
CV ≤5.0%
Ct ≤36
LoD 103CFU/mL
Te motuhaketanga Kāore he tauhohenga whakawhiti ki ētahi atu mate manawa pērā i te klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus influenzae, A. junii, A. haemolyticus, legionella pneumophila, escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, respiratory adenovirus, kei roto rānei i ngā tauira he ira ātete-rongoa CTX, mecA, SME, Ngā tauira o SHV me TEM.
Ngā Taputapu e Pā Ana Pūnaha PCR Wā-Tūturu o Applied Biosystems 7500

Ngā Pūnaha PCR Wā-Tūturu SLAN-96P

Pūnaha PCR Wā-Tūturu LightCycler®480

Rere Mahi

Ngā matū tango e taunakitia ana: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).


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