Zika Virus

Whakaahuatanga Poto:

Ka whakamahia tenei kete ki te kite i te tohu Zika huaketo nucleic acid i roto i nga tauira serum o nga turoro e whakapaehia ana he mate huaketo Zika in vitro.


Taipitopito Hua

Tohu Hua

ingoa hua

HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Tiwhikete

CE

Epidemiology

Ko te huaketo Zika no te puninga Flaviviridae, he wheori RNA pai-paetahi kotahi me te diameter o te 40-70nm.He kopaki kei roto, e 10794 nga nucleotides, e 3419 nga waikawa amino.E ai ki te momo iraira, ka wehewehea ki te momo Afirika me te momo Ahia.Ko te mate huaketo Zika he mate hopuhopu whakapeka whakapeka i a ia ano na te huaketo Zika, ka tukuna te nuinga ma te ngau a te waeroa Aedes aegypti.Ko te nuinga o nga ahuatanga haumanu ko te kirikaa, te ponana, te arthralgia, te conjunctivitis ranei, a he uaua ka mate.E ai ki te Whakahaere Hauora o te Ao, ko te microcephaly neonatal me te Guillain-Barre syndrome (Guillain-Barré syndrome) pea e pa ana ki te mate huaketo Zika.

Hongere

FAM Zika huaketo nucleic acid
ROX

Mana o roto

Tawhā Hangarau

Rokiroki ≤30 ℃ & parea i te marama
Papa-ora 12 marama
Momo Tauira serum hou
Ct ≤38
CV ≤5.0%
LoD 500 ng/μL
Tauwhāiti Ko nga hua whakamatautau ka puta mai i tenei kete karekau e pa ki te hemoglobin (<800g/L), bilirubin (<700μmol/L), me nga lipids toto/ triglycerides (<7mmol/L) i roto i te toto.
Nga taputapu e tika ana ABI 7500 Pūnaha PCR Wā-tūturu

ABI 7500 Pūnaha PCR Wā-tūturu Tere

QuantStudio®5 Pūnaha PCR Wā-tūturu

SLAN-96P Pūnaha PCR Wā-tūturu

Kaihurihuri®480 Pūnaha PCR Wā-tūturu

LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu

MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha

BioRad CFX96 Pūnaha PCR Wā-tūturu, BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu

Rere Mahi

Kōwhiringa 1.

QIAamp Viral RNA Mini Kit(52904), Tangohanga Waikawa Nucleic, Reagent Purification ranei(YDP315-R) na Tiangen Biotech(Beijing) Co., Ltd.Te tangohangakia tangohia kia rite ki nga tohutohu tangohanga, me te rōrahi tangohanga tūtohu ko 140 μL me te rōrahi elution tūtohu ko 60 μL.

Kōwhiringa 2.

Tonotono & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Me tango te tangohanga kia rite ki nga tohutohu.Ko te rahinga tauira tangohanga he 200 μL , a ko te 80μL te rōrahi elution tūtohutia.


  • Tōmua:
  • Panuku:

  • Tuhia to korero ki konei ka tukuna mai ki a matou