Staphylococcus Aureus and methicillin-ātete Staphylococcus Aureus (MRSA / SA)
Ingoa Hua
Hwts-ot062 staphylococcus aureus and methicillin-ātete staphylococcus Aureus (MRSA / SA) Kitsacic Kite (PCORESCENTENTCE PCR)
Tiwihikete
CE
Manganga
Staphylococcus Aureus Ko tetahi o nga huakita pathogen nui o te mate o te mate o te mate. Ko te Staphylococcus Aureus (SA) no te Staphylococcus he māngai no te huakita Gram-pai, ka taea te whakaputa i nga momo paitini me nga whakauru whakaeke. Kei te huakita nga ahuatanga o te tohatoha whanui, kaha kaha me te reiti whakaeke nui. Ko te ira numaero te waikawa (NUC) he ira tino pai o te staphylococcus aora.
Takiwā rerenga
| Whānau | Methicillin-ātete MAA GEN |
| Rox | Te mana o roto |
| Cy5 | Staphylococcus Aureus Nuku Gene |
Tohu hangarau
| Pākoro | ≤-18 ℃ & Ka tiakina mai i te maama |
| Te Haahi-Ora | 12 marama |
| Momo tauira | Sputum, kiri me nga tauira ngoikore o te kiko ngoikore, me nga tauira Spabs o NASAL |
| Ct | ≤36 |
| CV | ≤5.0% |
| Koropū | 1000 cfu / ml staphylococcus aureka, 1000 cfu / ml methigillin-ātete. Ka kitea e te kete te tohutoro a National Lod, 1000 / ML Staphylococcus Aureus Ka taea te kitea |
| Whakawhāititanga | Ko te whakamatautauranga whakawhiti, escherichia cosi, Klebsiella Pneumonia, te acinesbacter Baumannii, Proteus Milabilis, Incobacter Cloacae, Enterecoccus Faecimium, Candionalla Pneumoophila, Depeionalla pneumumhosis, legida suparshais, Neisseria meningitidis, Aemophilus Influenzae. |
| Nga taputapu e tika ana | Te tono Biosystems 7500 nga punaha PCR-tūturu Āneni®5 pūnaha procr pocr tūturu Slan-96P Pūnaha PCR tūturu Tairapa®480 Pūnaha PCR Real Ko te Pūnaha Tango-Real PCR Ma-6000 Motuhake TheMal Thermal Thermal Biorad CFX96 Pūnaha Real-Time Biorad CFX Opus 96 Pūnaha Real-Time |
Rere mahi
Kōwhiringa 1.
Macro & Micro-RNA Kit (HWS-3019) na Jiangsu Macro & Midd-Add Ka taea te whakamahi me te Macro & MODO-3006C, HWTS-3006C, HWTS-3006C 3006b). Tāpirihia te 200μl o te saline noa ki te ua, a me tango nga mahi e whai ake nei kia rite ki nga tohutohu, ko te kohinga tino pai ko te 80μl.
Kōwhiringa 2.
Macro & Whakamatau Whakamatau Tauira Whakamatautau (HWSS-3005-8) na Jiangsu Macro & Mid-Add-Tāpiri 1ml o te tote noa ki te horoi me te pai. Centrifuge i te 13,000r / min mo te 5 meneti, tangohia te supernatamant (rahui 10-20μl o te rangatira), me te whai i nga tohutohu mo te tangohanga o muri.
Whakahoutanga Tangohanga: Te tangohanga waikawa waikawa, te horoi ranei i te tuku (YDP302) na Tiangen Biotech (Beijing) Co., Ltd. Me kawe te tangohanga kia rite ki te taahiraa 2 o te pukapuka tohutohu. E taunaki ana kia whakamahia te Rnase me te wai koreutu-kore hei whakatikatika i te rōrahi 100μl.
