Kete Kimi Waikawa Nukleika Staphylococcus Aureus me te Staphylococcus Aureus Ātete-Methicillin
Ingoa hua
Kete Kimi Waikawa Nucleic Staphylococcus Aureus me te Staphylococcus Aureus Ātete-Methicillin (PCR Fluorescence)
Te Whakamahinga i Whakaritea
E whakamahia ana tēnei kete mō te kimi kounga o te staphylococcus aureus me ngā waikawa nucleic staphylococcus aureus ātete-methicillin i roto i ngā tauira huware tangata, ngā tauira ūkui ihu me ngā tauira mate kiri me ngā kiko ngohengohe i roto i te vitro. Ka whakamahia te tauira huware me te whakamātautau tauira ūkui ihu i roto i ngā whare hauora hei ārai me te whakahaere i te mate MRSA i roto i ngā tūroro hōhipera, tae atu ki ngā tūroro tiaki nui, ngā tūroro pokanga me ngā tūroro tiaki roa; Ka taea hoki te whakamahi mō te tātaritanga āwhina o ngā tūroro mate manawa Staphylococcus Aureus me te Methicillin-Resistant Staphylococcus Aureus; Me te hono atu rānei ki ētahi atu whakamātautau taiwhanga pērā i ngā ahurea moroiti hei āwhina i te tātaritanga o ngā mate kiri me ngā kiko ngohengohe MRSA/SA. Kāore ngā hua whakamātautau e whakamahia tika ana hei ārahi i te whakamahinga paturopi.
Te mate urutā
Ko te Staphylococcus aureus tētahi o ngā huakita mate nui o te mate pāwera i roto i te whare. Nō te staphylococcus te Staphylococcus aureus (SA), ā, he tohu ia nō ngā huakita Gram-pai, ka taea te whakaputa i ngā momo paitini me ngā whākōkī whakaeke. He whānui te horapa o ngā huakita, he kaha te mate, ā, he tere ātete teitei. Ko te ira nuclease werawera (nuc) he ira tino tiakina o te staphylococcus aureus.
I ngā tau tata nei, nā te whakamahinga nui o ngā homoni me ngā rongoā ārai mate, me te whakamahi kino i ngā paturopi whānui, kua piki haere ngā mate pāwera i roto i te whare i puta mai i te Methicillin-resistant Staphylococcus aureus (MRSA) i roto i te Staphylococcus. Ko te reiti kitea o te MRSA i te tau 2019 i Haina he 30.2% te toharite ā-motu. Kua wehea te MRSA ki te MRSA e pā ana ki te hauora (HA-MRSA), te MRSA e pā ana ki te hapori (CA-MRSA), me te MRSA e pā ana ki te kararehe (LA-MRSA). He rerekētanga nui ngā CA-MRSA, HA-MRSA, LA-MRSA i roto i te koiora moroiti, te ātete huakita (hei tauira, he nui ake te ātete o te HA-MRSA ki ngā rongoā maha i te CA-MRSA) me ngā āhuatanga haumanu (hei tauira, te wāhi pangia). E ai ki ēnei āhuatanga, ka taea te wehewehe i te CA-MRSA me te HA-MRSA. Heoi, kei te whakawhāiti haere ngā rerekētanga i waenga i te CA-MRSA me te HA-MRSA nā te nekehanga tonu o te tangata i waenga i ngā hōhipera me ngā hapori. He ātete maha ngā rongoā a te MRSA, ehara i te mea ko te ātete anake ki ngā paturopi β-lactam, engari ki ngā aminoglycosides, macrolides, tetracyclines me ngā quinolones hoki i ngā taumata rerekē. He rerekē ngā rohe o ngā reiti ātete rongoā me ngā au rerekē. Ka taea e te MRSA te uru ki te tinana mā roto i ngā patunga kiri, ngā pūtake makawe, te toto me ētahi atu take mate pukupuku, ā, he tino ngawari te pangia e te mate MRSA e ngā tūroro he mate kiri me ngā wera. Ko te niumōnia tētahi o ngā mate haumanu tino noa o te MRSA, ā, ko te maimoatanga kore tika he take nui o te mate nui.
Ngā Tawhā Hangarau
| Rokiroki | ≤ -18℃ |
| Te roa o te oranga | 12 marama |
| Momo Tauira | Hupa, ūkui ihu, tauira mate kiri me ngā kiko ngohengohe, |
| CV | <5.0% |
| LoD | 1000 CFU/mL |
| Te motuhaketanga | E whakaatu ana te whakamātautau tauhohenga-whakawhiti kāore he tauhohenga-whakawhiti o tēnei kete ki ētahi atu mate manawa pērā i te methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Ngā Taputapu e Pā Ana | Momo Tuatahi: Ngā Pūnaha PCR Wā-Tūturu a Biosystems 7500, Ngā Pūnaha PCR Wā-Tūturu a QuantStudio®5, Ngā Pūnaha PCR Wā-Tūturu a SLAN-96P (Hongshi Medical Technology Co., Ltd.), Pūnaha Kitenga PCR Wā-Tūturu a LineGene 9600 Plus (FQD-96A, hangarau Hangzhou Bioer), MA-6000 Kaihurihuri Wera Tau Wā-Tūturu (Suzhou Molarray Co., Ltd.), Pūnaha PCR Wā-Tūturu a BioRad CFX96, Pūnaha PCR Wā-Tūturu a BioRad CFX Opus 96. Momo Tuarua: HWTS-EudemonTM AIO800 |
Rere Mahi
Me whakarato ngā tauhohenga me ngā taputapu, engari kāore i te whakaratohia
Momo I
(1) Kete Whakamātautau Ira/RNA Whānui Macro & Micro-Test (HWTS-3019) mai i Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (Ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, 3006C).
(2)Tauira waiwhakarewa: 4% NaOH, Sputasol rānei mai i Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Te tote noa.
Momo Tuarua:
Kete Whakamātautau Ira/RNA Whānui mō te Macro & Micro-Test (HWTS-3019-8) mai i Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ngā mea e hiahiatia ana engari kāore i te whakaratohia:Ngā Matau Kore-RNase/DNase, Ngā karapu whakakorekore, Ngā ngongo EP Kore-RNase/DNase, Ngā rīpene ngongo-8 mō te PCR.
