Staphylococcus Aureus me te Staphylococcus Aureus Ātete-Methicillin (MRSA/SA)
Ingoa hua
HWTS-OT062 Kete Kimi Waikawa Nucleic Staphylococcus Aureus me te Staphylococcus Aureus Ātete-Methicillin (MRSA/SA) (PCR Fluorescence)
Tiwhikete
CE
Te mate urutā
Ko te Staphylococcus aureus tētahi o ngā huakita mate nui o te mate pāwera i roto i te whare. Nō te staphylococcus te Staphylococcus aureus (SA), ā, he tohu ia nō ngā huakita Gram-pai, ka taea te whakaputa i ngā momo paitini me ngā whākōkī whakaeke. He whānui te horapa o ngā huakita, he kaha te mate, ā, he tere ātete teitei. Ko te ira nuclease werawera (nuc) he ira tino tiakina o te staphylococcus aureus.
Hongere
| FAM | ira mecA ātete-methicillin |
| ROX | Mana Whakahaere ā-roto |
| CY5 | ira matua o te staphylococcus aureus |
Ngā Tawhā Hangarau
| Rokiroki | ≤-18℃ me te tiakina mai i te mārama |
| Te roa o te oranga | 12 marama |
| Momo Tauira | ngā tauira mate huware, kiri me ngā kiko ngohengohe, me ngā tauira ūkui ihu |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL huakita ātete-methicillin. Ina kitea e te kete te tohutoro LoD ā-motu, ka taea te kite i te 1000/mL staphylococcus aureus |
| Te motuhaketanga | E whakaatu ana te whakamātautau tauhohenga-whakawhiti kāore he tauhohenga-whakawhiti o tēnei kete ki ētahi atu mate manawa pērā i te methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha Koiora Whakamahia 7500 Ngā Pūnaha PCR Wā-Tūturu QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1.
Ka taea te whakamahi i te Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Tāpirihia he 200µL o te tote noa ki te matū kua tukatukahia, ā, me tango ngā mahi e whai ake nei kia rite ki ngā tohutohu, ā, ko te rōrahi elution kua tūtohutia he 80µL.
Kōwhiringa 2.
Te Whakamātautau Tauira Macro & Micro-Test (HWTS-3005-8) nā Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tāpirihia te 1mL o te tote noa ki te wai i muri i te horoinga ki te tote noa, kātahi ka whakaohokia kia pai. Whakakotahitia ki te 13,000r/min mō te 5 meneti, tangohia te wai i roto i te ipu (waiho kia 10-20µL o te wai i roto i te ipu), ā, whai i ngā tohutohu mō te tangohanga i muri mai.
Te matū tango e taunakitia ana: Te matū tango waikawa karihi, te matū horoi rānei (YDP302) nā Tiangen Biotech (Beijing) Co.,Ltd. Me mahi te tango kia rite tonu ki te taahiraa 2 o te pukapuka tohutohu. E taunakitia ana kia whakamahia te wai kore-RNase me te wai kore-DNase mō te whakawairakau me te rōrahi o te 100µL.
