Staphylococcus Aureus me te Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
ingoa hua
HWTS-OT062 Staphylococcus Aureus me te Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Kete Rapu Waikawa Nucleic(Fluorescence PCR)
Tiwhikete
CE
Epidemiology
Ko Staphylococcus aureus tetahi o nga huakita pathogenic nui o te mate nosocomial.Ko te Staphylococcus aureus (SA) no te staphylococcus, he kanohi mo nga huakita Gram-positive, ka taea te whakaputa i nga momo paitini me nga whākōkī urutomo.Ko nga huakita nga ahuatanga o te tohatoha whanui, te kaha o te pathogenicity me te tere o te aukati.Ko te ira karihi Thermotable (nuc) he ira tino tiakina o staphylococcus aureus.
Hongere
| FAM | ira mecA ātete-methicillin |
| ROX | Mana o roto |
| CY5 | staphylococcus aureus ira nuc |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ & parea i te marama |
| Papa-ora | 12 marama |
| Momo Tauira | te huhu, nga tauira mate kiri me te kiko ngohe, me nga tauira pania ihu |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL huakita ātete-methicillin.Ina kitea e te kete te tohutoro LoD a-motu, ka kitea te 1000/mL staphylococcus aureus. |
| Tauwhāiti | E whakaatu ana te whakamatautau tauhohenga whakawhiti karekau he tauhohenga whiti o tenei kete ki etahi atu tukumate manawa penei i te methicillin-tairongo staphylococcus aureus, coagulase-kino staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiumaniellaprotete mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Nga taputapu e tika ana | Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
Tonotono & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ka taea te whakamahi me te Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Taapirihia te 200µL o te tote noa ki te parapara kua oti te tukatuka, me te tango i nga mahi ka whai ake i runga i nga tohutohu, a ko te 80µL te rōrahi elution e taunaki ana.
Kōwhiringa 2.
Tonotono & Micro-Test Tauira Tuku Reagent (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Taapirihia te 1mL o te tote noa ki te para i muri i te horoinga ki te tote noa, ka uru pai.Whakanuia ki te 13,000r/min mo te 5 meneti, tangohia te supernatant (rahui 10-20µL o te supernatant), ka whai i nga tohutohu mo te tangohanga o muri.
Reagent tangohanga Manakohia: Tangohanga Acid Nucleic or Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd.Ko te tangohanga me tino rite ki te taahiraa 2 o te pukapuka tohutohu.E taunaki ana kia whakamahia te wai RNase me te DNase-kore mo te whakamaarama me te rahi o te 100µL.
