Zika Virus
ingoa hua
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Tiwhikete
CE
Epidemiology
Ko te huaketo Zika no te puninga Flaviviridae, he wheori RNA pai-paetahi kotahi me te diameter o te 40-70nm. He kopaki kei roto, e 10794 nga nucleotides, e 3419 nga waikawa amino. E ai ki te momo iraira, ka wehewehea ki te momo Afirika me te momo Ahia. Ko te mate huaketo Zika he mate hopuhopu whakapeka whakapeka i a ia ano na te huaketo Zika, ka tukuna te nuinga ma te ngau a te waeroa Aedes aegypti. Ko te nuinga o nga ahuatanga haumanu ko te kirikaa, te ponana, te arthralgia, te conjunctivitis ranei, a he uaua ka mate. E ai ki te Whakahaere Hauora o te Ao, ko te microcephaly neonatal me te Guillain-Barre syndrome (Guillain-Barré syndrome) ka hono pea ki te mate huaketo Zika.
Hongere
| FAM | Zika huaketo nucleic acid |
| ROX | Mana o roto |
Tawhā Hangarau
| Rokiroki | ≤30 ℃ & parea i te marama |
| Papa-ora | 12 marama |
| Momo Tauira | serum hou |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500Kape/mL |
| Tauwhāiti | Whakamahia te kete ki te kite i nga tauira serum ki te huaketo Zika kino, a he kino nga hua. Ko nga hua whakamatautau pokanoa e whakaatu ana ka kore e nui ake te kukū o te bilirubin i roto i te serum i te 168.2μmol / ml, ko te kukume hemoglobin i puta mai i te hemolysis kaore i neke ake i te 130g / L, ko te toto lipid toto e kore e neke atu i te 65mmol / ml, te tapeke IgG i roto i te serum kaua e neke ake i te 5mg / mL, kaore he paanga ki te huaketo chikunguka dengue ranei. Hepatitis A, Hepatitis B, Hepatitis C, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile huaketo me nga tauira serum genomic tangata ka tohua mo te whakamatautau whakawhiti-reactivity, a ko nga hua e whakaatu ana kaore he tauhohenga whakawhiti i waenga i tenei kete me nga tukumate kua whakahuahia i runga ake nei. |
| Nga taputapu e tika ana | ABI 7500 Pūnaha PCR Wā-tūturuABI 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu Kaihurihuri®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Kōwhiringa 1.
QIAamp Viral RNA Mini Kit(52904), Tangohanga Waikawa Nucleic, Reagent Purification ranei(YDP315-R) na Tiangen Biotech(Beijing) Co., Ltd.Te tangohangakia tangohia kia rite ki nga tohutohu tangohanga, me te rōrahi tangohanga tūtohu ko 140 μL me te rōrahi elution tūtohu ko 60 μL.
Kōwhiringa 2.
Tonotono & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Tonotono & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Me tango te tangohanga kia rite ki nga tohutohu. Ko te rahinga tauira tangohanga he 200 μL , a ko te 80μL te rōrahi elution tūtohutia.
