Huaketo Zika
Ingoa hua
Kete Kimi Waikawa Matū HWTS-FE002 mō te Huaketo Zika (PCR Whakapūngao)
Tiwhikete
CE
Te mate urutā
Nō te puninga Flaviviridae te huaketo Zika, he huaketo RNA kotahi-aho, he pai-aho, he whānui 40-70 nm. He kōpaki tōna, kei roto 10794 ngā nucleotide, ā, e 3419 ngā waikawa amino e whakamārama ana. E ai ki te momo ira, ka wehea ki te momo Āwherika me te momo Āhia. Ko te mate huaketo Zika he mate pāwera whakapeka-whaiaro i puta mai i te huaketo Zika, e horahia ana mā te ngau o ngā waeroa Aedes aegypti. Ko ngā āhuatanga haumanu ko te kirika, te ponana, te mamae o te hononga o te waewae, te conjunctivitis rānei, ā, he onge te mate. E ai ki te World Health Organization, tera pea he hononga o te neonatal microcephaly me te mate Guillain-Barre (Guillain-Barré syndrome) ki te mate huaketo Zika.
Hongere
| FAM | Te waikawa karihi o te huaketo Zika |
| ROX | Mana Whakahaere ā-roto |
Ngā Tawhā Hangarau
| Rokiroki | ≤30℃ me te tiaki mai i te mārama |
| Te roa o te oranga | 12 marama |
| Momo Tauira | serum hou |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Ngā Tārua/mL |
| Te motuhaketanga | Whakamahia te kete hei kimi i ngā tauira toto me te huaketo Zika kino, ā, he kino ngā hua. E whakaatu ana ngā hua o te whakamātautau pokanoa ina kore te kukū o te bilirubin i roto i te toto e neke atu i te 168.2μmol/ml, ko te kukū hemoglobin i puta mai i te hemolysis kāore e neke atu i te 130g/L, ko te kukū lipid toto kāore e neke atu i te 65mmol/ml, ko te kukū IgG katoa i roto i te toto kāore e neke atu i te 5mg/mL, kāore he pānga ki te kimi i te huaketo dengue, te huaketo Zika, te huaketo chikungunya rānei. Ko te huaketo Hepatitis A, te huaketo Hepatitis B, te huaketo Hepatitis C, te huaketo Herpes, te huaketo encephalitis hoiho rawhiti, te huaketo Hantavirus, te huaketo Bunya, te huaketo West Nile me ngā tauira toto ira tangata ka tīpakohia mō te whakamātautau tauhohenga whakawhiti, ā, e whakaatu ana ngā hua kāore he tauhohenga whakawhiti i waenga i tēnei kete me ngā mate pukupuku kua whakahuatia i runga ake nei. |
| Ngā Taputapu e Pā Ana | Ngā Pūnaha PCR Wā-Tūturu ABI 7500Ngā Pūnaha PCR Tere Wā-Tūturu ABI 7500 QuantStudio®5 Ngā Pūnaha PCR Wā-Tūturu Ngā Pūnaha PCR Wā-Tūturu SLAN-96P LightCycler®Pūnaha PCR Wā-Tūturu 480 Pūnaha Kitenga PCR Wā-Tūturu LineGene 9600 Plus MA-6000 Pūrere Huringa Wera Wā-Tūturu Pūnaha PCR Wā-Tūturu BioRad CFX96 Pūnaha PCR Wā-Tūturu BioRad CFX Opus 96 |
Rere Mahi
Kōwhiringa 1.
Kete Iti RNA Huakita QIAamp (52904), Kaiwhakaora Tango Waikawa Karihi, Kaiwhakaora Purei rānei (YDP315-R) nā Tiangen Biotech (Beijing) Co.,Ltd.Te tangohangame tango kia rite ki ngā tohutohu tango, ā, ko te rōrahi tango e taunakitia ana ko 140 μL, ā, ko te rōrahi wairewa e taunakitia ana ko 60 μL.
Kōwhiringa 2.
Kete Whakamātautau Makro me te Iti mō te Ira/RNA Huakita (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Pūwero Waikawa Nukleika Aunoa mō te Whakamātautau Makro me te Iti (HWTS-3006). Me tango te tangohanga kia rite ki ngā tohutohu. Ko te rōrahi tauira tangohanga he 200 μL, ā, ko te rōrahi wairewa kua tūtohutia he 80μL.
